| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 5; Tlr5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Tlr5 recombinant protein (Position: Q81-D766). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes toll-like receptor 5 (gene Tlr5) in mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 97.6 kDa, and the supplier reports an observed band at about 100 kDa.
Toll-like receptor 5 (TLR5) is a cell surface pattern recognition receptor that recognizes flagellin, the main protein of bacterial flagella. It signals through MyD88 to activate NF-κB and inflammatory gene expression and is prominent on intestinal epithelial and immune cells. TLR5 is studied in mucosal immunity, gut microbiota and bacterial infection.
| Target | Toll-like receptor 5 (gene Tlr5; UniProt Q9JLF7, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 97.6 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Tlr5 fragment (Gln81–Asp766), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places toll-like receptor 5 in the membrane. Tissue expression noted by UniProt (mouse): Highly expressed in liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tong Xing et al. (2020) Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy. Journal of the Science of Food and Agriculture. 10.1002/jsfa.10641
