| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transmembrane 6 superfamily member 2; TM6SF2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TM6SF2 recombinant protein (Position: D78-H377). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transmembrane 6 superfamily member 2 (gene TM6SF2) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 42.6 kDa, and the supplier reports an observed band at about 43 kDa.
TM6SF2 is a multi-pass membrane protein of the endoplasmic reticulum and ER–Golgi compartment in liver and intestine. It supports the lipidation and secretion of triglyceride-rich VLDL particles, and its loss leads to fat accumulation in hepatocytes. A common TM6SF2 variant (E167K) is a well-established genetic risk factor for fatty liver disease, making it a focus of hepatic lipid research.
| Target | Transmembrane 6 superfamily member 2 (gene TM6SF2; UniProt Q9BZW4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.6 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat RH35 cells, mouse liver tissue, mouse small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TM6SF2 fragment (Asp78–His377), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transmembrane 6 superfamily member 2 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Substantial expression in liver and intestine, whereas all other tissues analyzed show low levels. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
