| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TMEM166/EVA1A, which shares 66.7% and 50% amino acid (aa) sequence identity with mouse and rat TMEM166/EVA1A, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of EVA1A in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TMEM166/EVA1A Antibody Picoband® catalog # A11580-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human TMEM166/EVA1A, which shares 66.7% and 50% amino acid (aa) sequence identity with mouse and rat TMEM166/EVA1A, respectively.
- Molecular weight context: reported MW: 21 kDa; calculated MW: nan
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, IHC-F, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
eva-1 homolog A, regulator of programmed cell death. Eva-1 homolog A (C. elegans) is a protein that in humans is encoded by the EVA1A gene. It belongs to the FAM176 family. This gene is mapped to 2p12. EVA1A, also called TMEM166, acts as a regulator of programmed cell death, mediating both autophagy and apoptosis. Functional note: Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis. Reported localization: Endoplasmic reticulum membrane, Single-pass membrane protein, Lysosome membrane. Expression/tissue context: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.
Research relevance and current trends
- Proteasome / Ubiquitin: Researchers commonly examine how EVA1A relates to this theme using model systems and orthogonal readouts.
- Proteolysis/Ubiquitin: Researchers commonly examine how EVA1A relates to this theme using model systems and orthogonal readouts.
- Ubiquitin E3 Enzymes: Researchers commonly examine how EVA1A relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative EVA1A levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of EVA1A across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
