| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TMEM166/EVA1A, which shares 66.7% and 50% amino acid (aa) sequence identity with mouse and rat TMEM166/EVA1A, respectively. |
| Molecular weight | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein eva-1 homolog A (gene EVA1A) in human, mouse and rat samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 17.5 kDa, and the supplier reports an observed band at about 21 kDa.
EVA1A (also called TMEM166) is a small transmembrane protein. It has been reported to promote both autophagy and apoptosis, and it is studied as a regulator of programmed cell death in development and cancer.
| Target | Protein eva-1 homolog A (gene EVA1A; UniProt Q9H8M9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 17.5 kDa |
| Observed MW | 21 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Flow cytometry | Not specified by the supplier |
|---|---|
| Immunohistochemistry | Not specified by the supplier |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
| Western blot | Not specified by the supplier |
Samples with a confirmed band (WB): human HepG2 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human renal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the N-terminal region of human TMEM166/EVA1A. Sequence identity with the mouse and rat orthologs: 66.7% and 50%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein eva-1 homolog A in the endoplasmic reticulum membrane and lysosome membrane. Tissue expression noted by UniProt (human): Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
