| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1; PYROXD1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human TMEM52B recombinant protein (Position: T22-N183). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TMEM52B Antibody Picoband® is an antibody reagent for detection of TMEM52B (pyridine nucleotide-disulphide oxidoreductase domain 1). Researchers commonly use anti-TMEM52B antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, Flow, ELISA).
Boster Bio Anti-TMEM52B Antibody Picoband® catalog # A17417-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: TMEM52B — Transmembrane protein 240 (pyridine nucleotide-disulphide oxidoreductase domain 1). Alternative names: Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1; PYROXD1
- Antibody format: Polyclonal; Rabbit IgG
- Species context: Host: Rabbit, Reactivity: Human,Monkey,Mouse,Rat
- Purification: Immunogen affinity purified.
- Immunogen: E.coli-derived human TMEM52B recombinant protein (Position: T22-N183).
- Molecular weight context: observed 24 kDa, calculated 24145 MW (reported)
- Provided application(s): WB, IHC, Flow, ELISA
These attributes help contextualize how the antibody is commonly selected (host/clonality/isotype/label) and how signals are interpreted across sample types and assay formats.
Biological background
Function: Involved in cellular response to oxidative stress.
Cellular localization: Nucleus. Sarcomere.
Tissue details: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Background: TMEM52B (Transmembrane Protein 52B, also known as C12orf59) is a Protein Coding gene. The TMEM52B gene, located on 12p13.2, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, and frog. 2 alternatively spliced human isoforms have been reported. The 183-amino acid protein has a reported mass of 20,002 daltons. The cellular localization is predicted to be membrane-associated. C12orf59 might act as a potential oncogenic protein in gastric cancer (GC). Researchers propose that the decreased C12orf59 expression status is a prognostic biomarker of ccRCC and cooperates with the loss of von Hippel-Lindau (VHL) all the while promoting renal carcinogenesis. 192 organisms have orthologs with human gene TMEM52B.
Cross reactivity: No cross-reactivity with other proteins.
Research relevance and current trends
- Quantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.
- Isoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.
- Context-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.
Common research applications
- Western blot (WB): compare relative target abundance and apparent size shifts (e.g., isoforms/PTMs) across conditions.
- Immunohistochemistry (IHC): assess distribution across tissue compartments and compare staining patterns between groups.
- Flow cytometry: quantify target-positive populations and compare shifts after stimulation or differentiation.
Across these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.
Notes for experimental interpretation
- Apparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.
- Species reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.
- Control concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO/KD, peptide competition, or independent assays) to support interpretation.
For antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
