Anti-TNF Receptor II/TNFRSF1B Antibody Picoband®

Overview

Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® is an antibody for TNFRSF1B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: TNFRSF1B (bestrophin 1); UniProt: P20333
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 75 kDa, calculated 51561 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® catalog # A01437-1.

Biological background

Biological context: Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate.

Expression and localization notes: cellular localization: Cell membrane., tissue context: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium..

Common research applications

• Western blotting (WB): Compare TNFRSF1B levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of TNFRSF1B in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify TNFRSF1B-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Tumor necrosis factor receptor 2 (TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors (TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55-kDa protein, and TNFR2, a 70-kDa protein, have been mapped to human chromosomes 12 (12pter-cen) and 1 (1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane.
• Tissue details: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
• Research category: Cell Adhesion Proteins,ECM Proteins,Neuroscience,Sensory System,Visual System

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.