| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor alpha-induced protein 3;TNF alpha-induced protein 3;3.4.19.12;6.3.2.-;OTU domain-containing protein 7C;Putative DNA-binding protein A20;Zinc finger protein A20;A20p50;A20p37;TNFAIP3;OTUD7C; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TNFAIP3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GCO-20 is a rabbit monoclonal antibody that recognizes tumor necrosis factor alpha-induced protein 3 (gene TNFAIP3) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 89.6 kDa, and the supplier reports an observed band at about 82 kDa.
A20 (TNFAIP3) is a ubiquitin-editing enzyme with both deubiquitinase and E3 ligase activities. Induced by TNF and other inflammatory signals, it removes activating ubiquitin chains from signaling proteins such as RIPK1 and TRAF6, turning off NF-κB signaling in a negative feedback loop. A20 is a key brake on inflammation, and its genetic variants are linked to autoimmune diseases.
| Target | Tumor necrosis factor alpha-induced protein 3 (gene TNFAIP3; UniProt P21580, human) |
|---|---|
| Clone | GCO-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 89.6 kDa |
| Observed MW | 82 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human TNFAIP3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tumor necrosis factor alpha-induced protein 3 in the cytoplasm, nucleus and lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huang Jiangju et al. (2019) miR-873a-5p Targets A20 to Facilitate Morphine Tolerance in Mice. Frontiers in Neuroscience. 10.3389/fnins.2019.00347
