Anti-TNFRSF14 Antibody

Overview

Anti-TNFRSF14 Antibody is an antibody for TNFRSF14 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: TNFRSF14 (Tumor necrosis factor receptor superfamily member 14); UniProt: Q92956
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 170 kDa, calculated 30392 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-TNFRSF14 Antibody Picoband® catalog # A02298.

Biological background

Biological context: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .

Expression and localization notes: cellular localization: Membrane ; Single-pass type I membrane protein ., tissue context: Widely expressed, with the highest expression in lung, spleen and thymus..

Common research applications

• Western blotting (WB): Compare TNFRSF14 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of TNFRSF14 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify TNFRSF14-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane ; Single-pass type I membrane protein .
• Tissue details: Widely expressed, with the highest expression in lung, spleen and thymus.
• Research category: Antiviral Signaling,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immune System Diseases,Immunology,Innate Immunity,Signal Transduction,TNF Superfamily

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.