| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tissue-type plasminogen activator;t-PA;t-plasminogen activator;tPA;3.4.21.68;Alteplase;Reteplase;Tissue-type plasminogen activator chain A;Tissue-type plasminogen activator chain B;PLAT; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TPA recombinant protein (Position: H366-P562). Human TPA shares 83% and 84% amino acid (aa) sequence identity with mouse and rat TPA, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tissue-type plasminogen activator (gene PLAT) in human samples and is validated for Western blot. The predicted molecular weight is 62.9 kDa, and the supplier reports an observed band at about 63–69 kDa.
Tissue-type plasminogen activator (tPA) is a serine protease that converts plasminogen into plasmin, the enzyme that breaks down fibrin clots. Released mainly by endothelial cells, it is a central regulator of fibrinolysis, also acts in the brain on synaptic plasticity, and recombinant tPA is a standard thrombolytic drug.
| Target | Tissue-type plasminogen activator (gene PLAT; UniProt P00750, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 62.9 kDa |
| Observed MW | 63–69 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human U87 cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TPA fragment (His366–Pro562), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 83% and 84%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes tissue-type plasminogen activator as a secreted protein. Tissue expression noted by UniProt (human): Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
