| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tripeptidyl-peptidase 1;TPP-1;3.4.14.9;Cell growth-inhibiting gene 1 protein;Lysosomal pepstatin-insensitive protease;LPIC;Tripeptidyl aminopeptidase;Tripeptidyl-peptidase I;TPP-I;TPP1;CLN2;GIG1, UNQ267/PRO304; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TPP1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TPP1 (Tripeptidyl-peptidase 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TPP1 Antibody Picoband® catalog # PB9899. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human TPP1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
- Molecular weight context: reported MW: 39 kDa; calculated MW: 61248 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tripeptidyl-peptidase 1; Tripeptidyl-peptidase 1. Tripeptidyl-peptidase 1, also known as Lysosomal pepstatin-insensitive protease, is an enzyme that in humans is encoded by the TPP1 gene. This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. Functional note: Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus (By similarity). . Reported localization: Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Expression/tissue context: Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
Research relevance and current trends
- Neurodegenerative Disease: Researchers commonly examine how TPP1 (Tripeptidyl-peptidase 1) relates to this theme using model systems and orthogonal readouts.
- Neurogenesis: Researchers commonly examine how TPP1 (Tripeptidyl-peptidase 1) relates to this theme using model systems and orthogonal readouts.
- Neurology Process: Researchers commonly examine how TPP1 (Tripeptidyl-peptidase 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TPP1 (Tripeptidyl-peptidase 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of TPP1 (Tripeptidyl-peptidase 1) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
