| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tripeptidyl-peptidase 1;TPP-1;3.4.14.9;Cell growth-inhibiting gene 1 protein;Lysosomal pepstatin-insensitive protease;LPIC;Tripeptidyl aminopeptidase;Tripeptidyl-peptidase I;TPP-I;TPP1;CLN2;GIG1, UNQ267/PRO304; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TPP1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tripeptidyl-peptidase 1 (gene TPP1) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 61.2 kDa, and the supplier reports an observed band at about 39 kDa.
Tripeptidyl-peptidase 1 (TPP1) is a lysosomal serine protease that removes tripeptides from the free N-terminus of small polypeptides during protein breakdown in lysosomes. Loss of TPP1 activity causes classic late-infantile neuronal ceroid lipofuscinosis (CLN2 disease), so it is studied in lysosomal storage disorders.
| Target | Tripeptidyl-peptidase 1 (gene TPP1; UniProt O14773, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 61.2 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human TPP1. It differs from the mouse sequence at six residues and from the rat sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tripeptidyl-peptidase 1 in the lysosome and melanosome. Tissue expression noted by UniProt (human): Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
