| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | TNF receptor-associated factor 5;RING finger protein 84;TRAF5;RNF84; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human TRAF5 recombinant protein (Position: K277-L557). Human TRAF5 shares 82.3% amino acid (aa) sequence identity with mouse TRAF5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TRAF5 (Transcription factor E2F3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TRAF5 Antibody Picoband® catalog # A03076-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human TRAF5 recombinant protein (Position: K277-L557). Human TRAF5 shares 82.3% amino acid (aa) sequence identity with mouse TRAF5. (reported region: K277-L557).
- Molecular weight context: reported MW: 64 kDa; calculated MW: 64406 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Transcription factor E2F3; TNF receptor-associated factor 5. TNF receptor-associated factor 5, also known as RNF84, is a protein that in humans is encoded by the TRAF5 gene. The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family. Fluorescence in situ hybridization confirmed the regional localization of human TRAF5 to chromosome 1q32.2-q32.3. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. It is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. It seems to be involved in apoptosis. Functional note: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. . Reported localization: Cytoplasm . Cytoplasm, cytosol . Expression/tissue context: Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.
Research relevance and current trends
- Cytokines: Researchers commonly examine how TRAF5 (Transcription factor E2F3) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how TRAF5 (Transcription factor E2F3) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how TRAF5 (Transcription factor E2F3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TRAF5 (Transcription factor E2F3) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
