| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor MafA;Pancreatic beta-cell-specific transcriptional activator;Transcription factor RIPE3b1;V-maf musculoaponeurotic fibrosarcoma oncogene homolog A;MAFA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MAFA, identical to the related mouse sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of MAFA (Transcription factor MafA) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Transcription factor MafA MAFA Antibody catalog # RP1066. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human MAFA, identical to the related mouse sequence.
- Molecular weight context: reported MW: 37 kDa; calculated MW: 36982 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Transcription factor MafA; Transcription factor MafA. MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators. Functional note: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. . Reported localization: Nucleus . Detected in nuclei of pancreas islet beta cells. .
Research relevance and current trends
- Co-Factors: Researchers commonly examine how MAFA (Transcription factor MafA) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how MAFA (Transcription factor MafA) relates to this theme using model systems and orthogonal readouts.
- Growth Factors/Hormones: Researchers commonly examine how MAFA (Transcription factor MafA) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative MAFA (Transcription factor MafA) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the bZIP family. Maf subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
