| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9;p90;CD71;Transferrin receptor protein 1, serum form;sTfR;TFRC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TFR |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOAA-20 is a rabbit monoclonal antibody that recognizes transferrin receptor protein 1 (gene TFRC) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 84.9 kDa, and the supplier reports an observed band at about 95 kDa.
Transferrin receptor 1 (TFRC, CD71) is a homodimeric membrane receptor that takes up iron-loaded transferrin by endocytosis, releases the iron in acidified endosomes and then recycles to the cell surface. It is essential for erythropoiesis, is highly expressed on dividing cells and is widely used as a proliferation and erythroid marker.
| Target | Transferrin receptor protein 1 (gene TFRC; UniProt P02786, human) |
|---|---|
| Clone | AOAA-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 84.9 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human U-87MG cells, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human TFR.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transferrin receptor protein 1 in the cell membrane and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhongying Du et al. (2026) Electroacupuncture Pretreatment Ameliorates Perioperative Neurocognitive Disorder in Aged Mice by Inhibiting Ferroptosis Through the SIRT1/NRF2/GPX4 Pathway. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.71021
Fan Yang et al. (2025) Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells. Experimental Cell Research. 10.1016/j.yexcr.2025.114848
Wang Yali et al. (2022) Effect of arsenite on the proteome of earthworms Eisenia fetida. Soil Ecology Letters. 10.1007/s42832-021-0126-y
