| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9;p90;CD71;Transferrin receptor protein 1, serum form;sTfR;TFRC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transferrin receptor protein 1 (gene TFRC) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 84.9 kDa, and the supplier reports an observed band at about 95 kDa.
Transferrin receptor 1 (TFRC, CD71) is a homodimeric membrane receptor that takes up iron-loaded transferrin by endocytosis, releases the iron in acidified endosomes and then recycles to the cell surface. It is essential for erythropoiesis, is highly expressed on dividing cells and is widely used as a proliferation and erythroid marker.
| Target | Transferrin receptor protein 1 (gene TFRC; UniProt P02786, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 84.9 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human HT1080 cells, rat thymus tissue, rat RAW264.7 cells, mouse SP2/0 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with confirmed staining (ICC/IF): human placenta tissue.
Samples with a confirmed signal (flow cytometry): SiHa cells, U87 cells.
Immunogen
Recombinant human TFRC fragment (Met1–Asn198), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 72% and 70%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transferrin receptor protein 1 in the cell membrane and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shengting Deng et al. (2024) New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters. Animal Nutrition. 10.1016/j.aninu.2024.05.006
Yingzheng Zhao et al. (2025) Dual-ligand nanoprobes for fluorescence and magnetic particle imaging-guided glioma surgical resection. Journal of Drug Delivery Science and Technology. 10.1016/j.jddst.2025.107911
Aijing Liu et al. (2024) Endonuclear Circ-calm4 regulates ferroptosis via a circR-Loop of the COMP gene in pulmonary artery smooth muscle cells. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176944
Jing Gao et al. (2024) Novel insights into immune-related genes associated with type 2 diabetes mellitus-related cognitive impairment. World Journal of Diabetes. 10.4239/wjd.v15.i4.735
Haochen Yu et al. (2019) Sulfasalazine‑induced ferroptosis in breast cancer cells is reduced by the inhibitory effect of estrogen receptor on the transferrin receptor. Oncology Reports. 10.3892/or.2019.7189
Tao Feng et al. (2025) The Combination of Celastrol and Curcumin Enhances the Antitumor Effect in Nasopharyngeal Carcinoma by Inducing Ferroptosis. Biological & Pharmaceutical Bulletin. 10.1248/bpb.b25-00053
