| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Serotransferrin;Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF;PRO1400; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Transferrin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TF antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DOH-20; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-Transferrin TF Rabbit Monoclonal Antibody catalog # M00094. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TF (Serotransferrin).
- Antibody format: Monoclonal; clone DOH-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TF (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transferrins are iron binding transport proteins which can bind two Fe (3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Reported cellular localization context: Secreted. Tissue expression notes (as provided): Expressed by the liver and secreted in plasma.
Research relevance and current trends
- Research context keywords from the source record include: Cell Type Markers,2339,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Tags & Cell Markers,Transcription,Transcription Factors,Tumor Associated.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate TF antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TF expression by Western blot in cell or tissue lysates, Detect TF in FFPE tissue sections by immunohistochemistry, Quantify TF-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 52 kDa; calculated MW: 77064 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 52 kDa
- Cellular localization (provided): Secreted.
- Tissue details (provided): Expressed by the liver and secreted in plasma.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
