| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serotransferrin;Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF;PRO1400; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Transferrin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DOH-20 is a rabbit monoclonal antibody that recognizes serotransferrin (gene TF) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 77.1 kDa, and the supplier reports an observed band at about 75 kDa.
Serotransferrin (TF) is the main iron-transport protein of blood plasma, made mostly by the liver. Each molecule binds two ferric iron ions and delivers them to cells through the transferrin receptor, and transferrin levels and saturation are standard measures in iron metabolism research.
| Target | Serotransferrin (gene TF; UniProt P02787, human) |
|---|---|
| Clone | DOH-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.1 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human serum.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human transferrin.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes serotransferrin as a secreted protein. Tissue expression noted by UniProt (human): Expressed by the liver and secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
