| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein-glutamine gamma-glutamyltransferase 2;2.3.2.13;Tissue transglutaminase;Transglutaminase C;TG (C);TGC;TGase C;Transglutaminase H;TGase H;Transglutaminase-2;TGase-2;TGM2; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TGM2, different from the related mouse sequence by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TGM2 (Protein-glutamine gamma-glutamyltransferase 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Transglutaminase 2/TGM2 Antibody Picoband® catalog # PB9448. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human TGM2, different from the related mouse sequence by five amino acids.
- Molecular weight context: reported MW: 77 kDa; calculated MW: 77329 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein-glutamine gamma-glutamyltransferase 2; Protein-glutamine gamma-glutamyltransferase 2. Tissue transglutaminase, also called TGC, is a 78-kDa, calcium dependent enzyme of the protein-glutamine gamma-glutamyltransferases family. By fluorescence in situ hybridization (FISH), this gene is mapped in 20q11.23. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Functional note: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Research relevance and current trends
- Amino Acid Metabolism: Researchers commonly examine how TGM2 (Protein-glutamine gamma-glutamyltransferase 2) relates to this theme using model systems and orthogonal readouts.
- Amino Acids: Researchers commonly examine how TGM2 (Protein-glutamine gamma-glutamyltransferase 2) relates to this theme using model systems and orthogonal readouts.
- Apoptosis: Researchers commonly examine how TGM2 (Protein-glutamine gamma-glutamyltransferase 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TGM2 (Protein-glutamine gamma-glutamyltransferase 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of TGM2 (Protein-glutamine gamma-glutamyltransferase 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the transglutaminase superfamily. Transglutaminase family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
