| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trem-like transcript 1 protein;TLT-1;Triggering receptor expressed on myeloid cells-like protein 1;TREML1;TLT1;UNQ1825/PRO3438; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TREML1 recombinant protein (Position: Q16-P162). Human TREML1 shares 69.3% amino acid (aa) sequence identity with mouse TREML1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects trem-like transcript 1 protein (gene TREML1) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 32.7 kDa, and the supplier reports an observed band at about 33 kDa.
TREM-like transcript 1 (TLT-1, TREML1) is a receptor of the TREM family stored in platelet α-granules and moved to the platelet surface on activation. It binds fibrinogen and supports platelet aggregation, and it is studied in hemostasis, thrombosis and the link between platelets and inflammation.
| Target | Trem-like transcript 1 protein (gene TREML1; UniProt Q86YW5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 32.7 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human TREML1 fragment (Gln16–Pro162), expressed in E. coli. Sequence identity with the mouse ortholog: 69.3%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places trem-like transcript 1 protein in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Detected in platelets, monocytic leukemia and in T-cell leukemia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
