| Field | Specification |
|---|---|
| Target | |
| Alternative names | Three-prime repair exonuclease 1;3.1.11.2;3'-5' exonuclease TREX1;DNase III;TREX1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TREX1, different from the related mouse sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes three-prime repair exonuclease 1 (gene TREX1) in human samples and is validated for Western blot. The predicted molecular weight is 33.2 kDa, and the supplier reports an observed band at about 33 kDa.
TREX1 is the main 3′-to-5′ DNA exonuclease in the cytoplasm of mammalian cells. By degrading DNA from retroelements, micronuclei and other sources, it prevents inappropriate activation of the cGAS–STING pathway and type I interferon production. TREX1 mutations cause Aicardi-Goutières syndrome and are linked to lupus, so it is central to nucleic acid sensing research.
| Target | Three-prime repair exonuclease 1 (gene TREX1; UniProt Q9NSU2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 33.2 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): SMMC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 40 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human TREX1. It differs from the mouse sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places three-prime repair exonuclease 1 in the nucleus, cytoplasm and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Detected in thymus, spleen, liver, brain, heart, small intestine and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
