| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Telomeric repeat-binding factor 1;NIMA-interacting protein 2;TTAGGG repeat-binding factor 1;Telomeric protein Pin2/TRF1;TERF1;PIN2, TRBF1, TRF, TRF1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human TRF1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TRF1 TERF1 Rabbit Monoclonal Antibody is an antibody targeting TERF1. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOIB-20; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 48 kDa; calculated MW: 50246 MW.
Boster Bio Anti-TRF1 TERF1 Rabbit Monoclonal Antibody catalog # M02474. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: TERF1 — Telomeric repeat-binding factor 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOIB-20; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 48 kDa; Calculated: 50246 MW
Biological background
Protein function (datasheet): Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double- stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. .
Cellular localization (datasheet): Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, telomere. Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.
Tissue details (datasheet): Highly expressed and ubiquitous. Isoform Pin2 predominates.
Research relevance and current trends
- Commonly studied in contexts related to Chromosome Structure,Epigenetics and Nuclear Signaling,Nucleus,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
