| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tribbles homolog 2;TRB-2;TRIB2 ;TRB2 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TRIB2, identical to the related mouse sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TRIB2 (Tribbles homolog 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TRIB2 Antibody Picoband® catalog # PB9900. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human TRIB2, identical to the related mouse sequence.
- Molecular weight context: reported MW: 39 kDa; calculated MW: 38801 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tribbles homolog 2; Tribbles homolog 2. Tribbles homolog 2 is a protein that in humans is encoded by the TRIB2 gene. This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBP alpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. Functional note: Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity (By similarity). . Reported localization: Cytoplasm . Cytoplasm, cytoskeleton . May associate with the cytoskeleton. . Expression/tissue context: Highly expressed in peripheral blood leukocytes. .
Research relevance and current trends
- Atherosclerosis: Researchers commonly examine how TRIB2 (Tribbles homolog 2) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how TRIB2 (Tribbles homolog 2) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how TRIB2 (Tribbles homolog 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TRIB2 (Tribbles homolog 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of TRIB2 (Tribbles homolog 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
