| Field | Specification |
|---|---|
| Target | |
| Alternative names | High affinity nerve growth factor receptor;2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;Trk-A;gp140trk;p140-TrkA;NTRK1;MTC, TRK, TRKA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TrkA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CDH-14 is a rabbit monoclonal antibody that recognizes high affinity nerve growth factor receptor (gene NTRK1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 87.5 kDa, and the supplier reports an observed band at about 145 kDa.
TrkA (NTRK1) is the high-affinity receptor tyrosine kinase for nerve growth factor (NGF). NGF binding triggers receptor autophosphorylation and activation of MAPK, PI3K/AKT and PLCγ signaling that supports the survival and differentiation of sensory and sympathetic neurons, and TrkA is studied in pain, neurodevelopment and NTRK fusion-driven cancers.
| Target | High affinity nerve growth factor receptor (gene NTRK1; UniProt P04629, human) |
|---|---|
| Clone | CDH-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 87.5 kDa |
| Observed MW | 145 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): human HEL cells, rat brain tissue, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human trkA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places high affinity nerve growth factor receptor in the cell membrane, early endosome membrane, late endosome membrane and recycling endosome membrane. Tissue expression noted by UniProt (human): Isoform TrkA-I is found in most non-neuronal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kan Bu-Fan et al. (2024) Nerve Growth Factor/Tyrosine Kinase A Receptor Pathway Enhances Analgesia in an Experimental Mouse Model of Bone Cancer Pain by Increasing Membrane Levels of δ-Opioid Receptors. Anesthesiology. 10.1097/ALN.0000000000004880
Lei Wang et al. (2006) Nerve Growth Factor and Tyrosine Kinase A in Human Salivary Adenoid Cystic Carcinoma: Expression Patterns and Effects on In Vitro Invasive Behavior. Journal of Oral and Maxillofacial Surgery. 10.1016/j.joms.2005.12.011
