| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA-binding protein RO60; 60 kDa SS-A/Ro ribonucleoprotein; 60 kDa Ro protein; 60 kDa ribonucleoprotein Ro; RoRNP; Ro 60 kDa autoantigen; Ro60 autoantigen; Sjoegren syndrome antigen A2; Sjoegren syndrome type A antigen; SS-A; TROVE domain family member 2; RO60; SSA2; TROVE2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TROVE2/SS-A/RO60 recombinant protein (Position: K88-M419). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets RNA-binding protein RO60 in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 60.7 kDa, and the supplier reports an observed band at about 60 kDa.
RO60 (also known as SS-A or TROVE2) is an RNA-binding protein that associates with small noncoding Y RNAs and binds misfolded noncoding RNAs, such as defective pre-5S rRNA, acting in RNA quality control. It is best known as a major target of autoantibodies in Sjögren syndrome and systemic lupus erythematosus.
| Target | RNA-binding protein RO60 (gene RO60; UniProt P10155, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.7 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human Jurkat cells, human 293T cells, human HeLa cells, rat thymus tissue, rat spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Daudi cells.
Immunogen
Recombinant human TROVE2/SS-A/RO60 fragment (Lys88–Met419), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places RNA-binding protein RO60 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
