Anti-TRPM2 Antibody Picoband®

Overview

Anti-TRPM2 Antibody Picoband® is an antibody for TRPM2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: TRPM2 (Complement component C9); UniProt: O94759
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 171 kDa, calculated 63173 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-TRPM2 Antibody Picoband® catalog # A01013-1.

Biological background

Biological context: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

Expression and localization notes: cellular localization: Secreted. Cell membrane; Multi-pass membrane protein. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore., tissue context: Plasma..

Common research applications

• Western blotting (WB): Compare TRPM2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of TRPM2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify TRPM2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Transient receptor potential cation channel subfamily M member 2, also known as TRPM2 is a protein that in humans is encoded by the TRPM2 gene. Using a cosmid/BAC contig, this gene is mapped to chromosome 21q22.3. The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted. Cell membrane; Multi-pass membrane protein. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
• Tissue details: Plasma.
• Research category: Complement,Immunology,Innate Immunity

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.