| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pre-mRNA-processing factor 19 ;6.3.2.- ;Nuclear matrix protein 200 ;PRP19/PSO4 homolog ;hPso4 ;Senescence evasion factor ;PRPF19 ;NMP200 , PRP19 , SNEV ; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TrpRS |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23W83 is a rabbit monoclonal antibody that recognizes tryptophan--tRNA ligase, cytoplasmic (gene WARS1) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 53.2 kDa, and the supplier reports an observed band at about 53 kDa.
WARS1 is the cytoplasmic tryptophanyl-tRNA synthetase, which attaches tryptophan to its tRNA for protein synthesis. It is strongly induced by interferon-γ, and alternatively spliced or cleaved fragments (such as T2-TrpRS) inhibit angiogenesis. WARS1 is studied in translation, immunity and vascular biology.
| Target | Tryptophan--tRNA ligase, cytoplasmic (gene WARS1; UniProt P23381, human) |
|---|---|
| Clone | 23W83 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.2 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human trpRS.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tryptophan--tRNA ligase, cytoplasmic in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
