| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily V member 5;TrpV5;Calcium transport protein 2;CaT2;Epithelial calcium channel 1;ECaC;ECaC1;Osm-9-like TRP channel 3;OTRPC3;TRPV5;ECAC1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TRPV5, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transient receptor potential cation channel subfamily V member 5 (gene TRPV5) in human and rat samples and is validated for Western blot. The predicted molecular weight is 82.6 kDa, and the supplier reports an observed band at about 83 kDa.
TRPV5 is a highly calcium-selective channel of the vanilloid TRP subfamily found mainly in the distal kidney tubule. It is constitutively open at low intracellular calcium and provides the entry step for active calcium reabsorption from urine, with calcium-dependent feedback limiting its activity. It can combine with TRPV6 and is studied in calcium homeostasis.
| Target | Transient receptor potential cation channel subfamily V member 5 (gene TRPV5; UniProt Q9NQA5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 82.6 kDa |
| Observed MW | 83 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat pancreas tissue, rat lung tissue, rat intestine tissue, SW620 cells, COLO320 cells, 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human TRPV5. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places transient receptor potential cation channel subfamily V member 5 in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
