| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thioredoxin;Trx;ATL-derived factor;ADF;Surface-associated sulphydryl protein;SASP;TXN;TRDX, TRX, TRX1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TRX1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOGB-20 is a rabbit monoclonal antibody that recognizes thioredoxin (gene TXN) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 11.7 kDa, and the supplier reports an observed band at about 12 kDa.
Thioredoxin (TXN, TRX1) is a small, ubiquitous oxidoreductase whose active-site pair of cysteines reduces disulfide bonds in other proteins. Kept reduced by thioredoxin reductase and NADPH, it protects cells against oxidative stress, supports enzymes such as ribonucleotide reductase and peroxiredoxins, and regulates transcription factors including NF-κB and AP-1.
| Target | Thioredoxin (gene TXN; UniProt P10599, human) |
|---|---|
| Clone | AOGB-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.7 kDa |
| Observed MW | 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human A549 cells, human HeLa cells, human HepG2 cells, human SIHA cells, rat liver tissue, rat RH35 cells, mouse liver tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TRX1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes thioredoxin as secreted and also located in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Lei et al. (2023) Macrophage-expressed SRA ameliorates alcohol-induced liver injury by suppressing S-glutathionylation of Notch1 via recruiting Thioredoxin. Journal of Leukocyte Biology. 10.1093/jleuko/qiad110
