| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Tryptophan Hydroxylase recombinant protein (Position: M1-K111). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tryptophan 5-hydroxylase 1 (gene TPH1) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 51.0 kDa, and the supplier reports an observed band at about 51 kDa.
Tryptophan hydroxylase 1 (TPH1) catalyzes the first and rate-limiting step of serotonin synthesis, converting L-tryptophan into 5-hydroxytryptophan. It is the isoform found mainly in peripheral tissues such as gut enterochromaffin cells and the pineal gland, so it is studied in gut serotonin signaling, platelet function and melatonin production.
| Target | Tryptophan 5-hydroxylase 1 (gene TPH1; UniProt P17752, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 51.0 kDa |
| Observed MW | 51 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SGC-7901 cells, rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Tryptophan Hydroxylase fragment (Met1–Lys111), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Seems to be less widely expressed than isoform 1. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yiwei Liao et al. (2026) Ligilactobacillus murinus alleviates binge-eating behavior by restoring serotonin metabolism. Food Research International. 10.1016/j.foodres.2026.118691
