Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®

Overview

Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® is an antibody for TPH1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: TPH1 (tryptophan hydroxylase 1); UniProt: P17752
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 60 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® catalog # A01626-4.

Biological background

Biological context: TPH1 is commonly studied as part of broader pathways controlling cellular state and phenotype. Researchers often assess changes in abundance, localization, or interaction partners across experimental conditions.

Expression and localization notes: tissue context: Isoform 2 seems to be less widely expressed than isoform 1..

Common research applications

• Western blotting (WB): Compare TPH1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of TPH1 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify TPH1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Tryptophan hydroxylase 1 (TPH1) is an isoenzyme of tryptophan hydroxylase which in humans is encoded by the TPH1 gene. This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.
• Cross reactivity: No cross-reactivity with other proteins.
• Tissue details: Isoform 2 seems to be less widely expressed than isoform 1.
• Research category: Amino Acid Metabolism,Amino Acids,Cancer,Cancer Metabolism,Endocrine Metabolism,Energy Metabolism,Energy Transfer Pathways,Hormone Biosynthesis,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Neuroscience,Neurotransmitter,Pathways and Processes,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.