| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RalA-binding protein 1;RalBP1;76 kDa Ral-interacting protein;Dinitrophenyl S-glutathione ATPase;DNP-SG ATPase;Ral-interacting protein 1;RALBP1;RLIP1, RLIP76; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human TXNIP |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TXNIP antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEBA-20; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-TXNIP Monoclonal Antibody catalog # M01409. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TXNIP (RalA-binding protein 1).
- Antibody format: Monoclonal; clone AEBA-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TXNIP (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S- (2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. . Reported cellular localization context: Membrane ; Peripheral membrane protein . Tissue expression notes (as provided): Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Metabolism,Cancer Susceptibility,Carbohydrate Metabolism,Cell Biology,Co-Activators/Co-Repressors,Energy Metabolism,Energy Transfer Pathways,Epigenetics and Nuclear Signaling,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism Of Carbohydrates,Nuclear Receptors,Nuclear Signaling Pathways,Oncoproteins/Suppressors,Oxidative Stress,Pathways and Processes,Redox Metabolism,Signal Transduction,Transcription,Tumor Suppressors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate TXNIP antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TXNIP expression by Western blot in cell or tissue lysates, Detect TXNIP in FFPE tissue sections by immunohistochemistry, Localize TXNIP by immunofluorescence/immunocytochemistry in cultured cells, Quantify TXNIP-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 42 kDa; calculated MW: 76063 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 42 kDa
- Cellular localization (provided): Membrane ; Peripheral membrane protein .
- Tissue details (provided): Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
