| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Thioredoxin reductase 2, mitochondrial;1.8.1.9;Selenoprotein Z;SelZ;TR-beta;Thioredoxin reductase TR3;TXNRD2;KIAA1652, TRXR2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TXNRD2, different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TXNRD2 Antibody Picoband® is an antibody targeting TXNRD2. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human,Rat; observed MW: 110 kDa; calculated MW: 56507 MW.
Boster Bio Anti-TXNRD2 Antibody catalog # PA1903. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: TXNRD2 — Thioredoxin reductase 2, mitochondrial
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human,Rat
- Molecular weight guidance: Observed: 110 kDa; Calculated: 56507 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox- regulated cell signaling.
Scientific background (datasheet): TXNRD2 (Thioredoxin reductase 2), also known as TRXR2, TR3, SELZ, or TR-BETA, Thioredoxin reductases, are selenocysteine (sec)-containing flavoenzymes that maintain thioredoxins, small proteins that catalyze redox reactions, in the reduced state using the reducing power of NADPH. By STS analysis and genomic sequence analysis, respectively, Miranda-Vizuete et al. (1999) and Sun et al. (1999) mapped the TXNRD2 gene to chromosome 22q11.2. Miranda-Vizuete et al. (1999) mapped the mouse gene to chromosome 16. Gasdaska et al. (1999) showed that TXNRD2 was a thioredoxin reductase that could ly reduce proteins such as insulin.
Cellular localization (datasheet): Mitochondrion .
Tissue details (datasheet): Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low. .
Sequence similarities (datasheet): Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Metabolism,Oxidative Stress,Pathways and Processes,Redox Metabolism.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
