| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thioredoxin reductase 2, mitochondrial;1.8.1.9;Selenoprotein Z;SelZ;TR-beta;Thioredoxin reductase TR3;TXNRD2;KIAA1652, TRXR2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TXNRD2, different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes thioredoxin reductase 2, mitochondrial (gene TXNRD2) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 56.5 kDa, and the supplier reports an observed band at about 110 kDa.
Thioredoxin reductase 2 (TXNRD2) is the mitochondrial selenoprotein that keeps mitochondrial thioredoxin (TXN2) reduced using NADPH. By supporting the mitochondrial thioredoxin system, it helps control reactive oxygen species and redox homeostasis within mitochondria.
| Target | Thioredoxin reductase 2, mitochondrial (gene TXNRD2; UniProt Q9NNW7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 56.5 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): rat kidney tissue, rat ovary tissue, rat liver tissue, SMMC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung cancer tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human TXNRD2. It differs from the rat sequence at three residues and from the mouse sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places thioredoxin reductase 2, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
