| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | D (1A) dopamine receptor;Dopamine D1 receptor;DRD1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human TYRO3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TYRO3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22T29; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-TYRO3 Rabbit Monoclonal Antibody catalog # M00913-5. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TYRO3 (D(1A) dopamine receptor).
- Antibody format: Monoclonal; clone 22T29; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TYRO3 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Reported cellular localization context: Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. . Tissue expression notes (as provided): Detected in caudate, nucleus accumbens and in the olfactory tubercle. .
Research relevance and current trends
- Research context keywords from the source record include: Neuroscience,Neurotransmission,Receptors / Channels.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate TYRO3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TYRO3 expression by Western blot in cell or tissue lysates, Detect TYRO3 in FFPE tissue sections by immunohistochemistry, Localize TYRO3 by immunofluorescence/immunocytochemistry in cultured cells, Enrich TYRO3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 120 kDa; calculated MW: 49293 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 120 kDa
- Cellular localization (provided): Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. .
- Tissue details (provided): Detected in caudate, nucleus accumbens and in the olfactory tubercle. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
