| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3-independent; E2 ubiquitin-conjugating enzyme; E2/E3 hybrid ubiquitin-protein ligase UBE2O; Ubiquitin carrier protein O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O; UBE2O; KIAA1734 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human UBE2O recombinant protein (Position: R150-A1283). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects (E3-independent) E2 ubiquitin-conjugating enzyme (gene UBE2O) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 141.3 kDa, and the supplier reports an observed band at about 200 kDa.
UBE2O is a large E2/E3 hybrid ubiquitin ligase that can transfer ubiquitin directly to substrates without a separate E3 enzyme. It monoubiquitinates targets such as the nuclear localization signal of BAP1 and SMAD6, and it is studied in erythroid maturation, protein quality control, NF-κB regulation and cancer.
| Target | (E3-independent) E2 ubiquitin-conjugating enzyme (gene UBE2O; UniProt Q9C0C9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 141.3 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human Jurkat cells, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Daudi cells.
Immunogen
Recombinant human UBE2O fragment (Arg150–Ala1283), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places (E3-independent) E2 ubiquitin-conjugating enzyme in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Predominantly expressed in skeletal muscle and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
