| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ubiquitin-conjugating enzyme E2 Q2;2.3.2.23 ;E2 ubiquitin-conjugating enzyme Q2;Ubiquitin carrier protein Q2;Ubiquitin-protein ligase Q2;UBE2Q2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human UBE2Q2, different from the related mouse sequence by four amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of UBE2Q2 (Ubiquitin-conjugating enzyme E2 Q2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-UBE2Q2 Antibody Picoband® catalog # PB9839. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human UBE2Q2, different from the related mouse sequence by four amino acids.
- Molecular weight context: reported MW: 50 kDa; calculated MW: 42818 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Ubiquitin-conjugating enzyme E2 Q2; Ubiquitin-conjugating enzyme E2 Q2. UBE2Q2 is identified as a putative ubiquitin-conjugating enzyme (E2) in a microarray screen for mitotic regulatory proteins. Its gene is mapped to 15q24.2. UBE2Q2 can covalently bind ubiquitin on the active site cysteine within the UBC domain. Inhibition of UBE2Q2 in HeLa cells causes an early mitotic arrest and increases cytotoxicity when the cells are treated with microtubule-inhibiting agents (MIAs). Changes in cell cycle progression and viability are not observed in the absence of MIA treatment, indicating that UBE2Q2 is involved in the response to MIAs rather than performing a more general function in mitosis. Moreover, inhibition of the UBE2Q2 protein causes cells to undergo a prolonged prophase arrest, suggesting that UBE2Q2 normally functions to antagonize an early mitotic checkpoint. Finally, inhibition of UBE2Q2 also sensitizes cells to the cytotoxic effects of MIAs through caspase-mediated apoptosis that is correlated with PARP1 cleavage. Functional note: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. . Reported localization: Cytoplasm . Expression/tissue context: Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium. .
Research relevance and current trends
- Chromatin Modifying Enzymes: Researchers commonly examine how UBE2Q2 (Ubiquitin-conjugating enzyme E2 Q2) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how UBE2Q2 (Ubiquitin-conjugating enzyme E2 Q2) relates to this theme using model systems and orthogonal readouts.
- Ubiquitin & Ubiquitin Like Modifiers: Researchers commonly examine how UBE2Q2 (Ubiquitin-conjugating enzyme E2 Q2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative UBE2Q2 (Ubiquitin-conjugating enzyme E2 Q2) levels across conditions; band patterns may reflect isoforms and processing.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
