| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin-conjugating enzyme E2 Q2;2.3.2.23 ;E2 ubiquitin-conjugating enzyme Q2;Ubiquitin carrier protein Q2;Ubiquitin-protein ligase Q2;UBE2Q2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human UBE2Q2, different from the related mouse sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ubiquitin-conjugating enzyme E2 Q2 (gene UBE2Q2) in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 42.8 kDa, and the supplier reports an observed band at about 50 kDa.
UBE2Q2 is a ubiquitin-conjugating (E2) enzyme that accepts ubiquitin from the E1 enzyme and can form Lys48-linked polyubiquitin chains in vitro. Its specific E3 partners and cellular roles have not been fully characterized.
| Target | Ubiquitin-conjugating enzyme E2 Q2 (gene UBE2Q2; UniProt Q8WVN8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.8 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human placenta tissue, human HeLa cells, human U87 cells, human 293T cells, rat stomach tissue, rat pancrease tissue, mouse pancrease tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the N-terminal region of human UBE2Q2. It differs from the mouse sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin-conjugating enzyme E2 Q2 in the cytoplasm. Tissue expression noted by UniProt (human): Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
