| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin conjugation factor E4 B; Homozygously deleted in neuroblastoma 1; RING-type E3 ubiquitin transferase E4 B; Ubiquitin fusion degradation protein 2; UBE4B; HDNB1; KIAA0684; UFD2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from UBE4B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26U59 is a rabbit monoclonal antibody that recognizes ubiquitin conjugation factor E4 B (gene UBE4B) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 146.2 kDa, and the supplier reports an observed band at about 130 kDa.
UBE4B is a U-box ubiquitin ligase that also works as an E4 chain-elongation factor, extending polyubiquitin chains on substrates. On early endosomes it helps ubiquitinate membrane cargo such as EGFR so that these proteins are sorted for lysosomal degradation. Animal studies link it to heart development and neuronal protection, and it is studied in protein turnover and neurodegeneration.
| Target | Ubiquitin conjugation factor E4 B (gene UBE4B; UniProt O95155, human) |
|---|---|
| Clone | 26U59 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 146.2 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293T cells, NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of UBE4B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin conjugation factor E4 B in the early endosome, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in differentiated myotubes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
