| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase UHRF1;6.3.2.-;Inverted CCAAT box-binding protein of 90 kDa;Nuclear protein 95;Nuclear zinc finger protein Np95;HuNp95;hNp95;RING finger protein 106;Transcription factor ICBP90;Ubiquitin-like PHD and RING finger domain-containing protein 1;hUHRF1;Ubiquitin-like-containing PHD and RING finger domains protein 1;UHRF1;ICBP90, NP95, RNF106; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human UHRF1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
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| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase UHRF1 in human samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 89.8 kDa, and the supplier reports an observed band at about 100 kDa.
UHRF1 is an epigenetic regulator and E3 ubiquitin ligase that is essential for maintaining DNA methylation through cell division. It binds hemimethylated DNA at replication forks and specific histone H3 marks, then ubiquitinates histone H3 to recruit DNMT1, which copies methylation onto the new strand. UHRF1 is studied in epigenetics, development and cancer.
| Target | E3 ubiquitin-protein ligase UHRF1 (gene UHRF1; UniProt Q96T88, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 89.8 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, human T47D cells, human A549 cells, human HepG2 cells, human MDA-MB-453 cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U2OS cells, A431 cells.
Immunogen
Synthetic peptide from the N-terminal region of human UHRF1. It differs from the mouse sequence at six residues and from the rat sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places E3 ubiquitin-protein ligase UHRF1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in thymus, bone marrow, testis, lung and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhenbo Tu et al. (2020) UHRF1 predicts poor prognosis by triggering cell cycle in lung adenocarcinoma. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.15438
