| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Urokinase plasminogen activator surface receptor; U-PAR; Monocyte activation antigen Mo3; uPAR; CD87; Plaur; MO3; UPAR |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human uPA Receptor/U-PAR/PLAUR recombinant protein (Position: E128-R167). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-uPA Receptor/U-PAR/PLAUR Antibody Picoband® is an antibody for PLAUR detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PLAUR (plasminogen activator, urokinase receptor); UniProt: Q03405
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 45 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-uPA Receptor/U-PAR/PLAUR Antibody Picoband® catalog # A00993-4.
Biological background
Biological context: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
Expression and localization notes: cellular localization: Cell membrane. invadopodium membrane. GPI-anchor. Secreted., tissue context: Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain..
Common research applications
- Western blotting (WB): Compare PLAUR levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PLAUR in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify PLAUR-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: PLAUR(PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. invadopodium membrane. GPI-anchor. Secreted.
- Tissue details: Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
- Research category: Immunology,Cancer,Cardiovascular,Cell Type Markers,Extracellular Matrix,Tumor Biomarkers,Invasion/Microenvironment,Receptors
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
