| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Uroplakin Ib/UPIb recombinant protein (Position: I107-Q216). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Uroplakin Ib/UPIb Antibody Picoband® is an antibody for UPK1B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: UPK1B (Protein NDRG3); UniProt: O75841
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 30 kDa, calculated 41409 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Uroplakin Ib/UPIb Antibody Picoband® catalog # A10230-1.
Biological background
Biological context: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
Expression and localization notes: cellular localization: Membrane ; Single-pass type I membrane protein ., tissue context: Ubiquitous. Highly expressed in brain. ..
Common research applications
- Western blotting (WB): Compare UPK1B levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of UPK1B in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify UPK1B-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Uroplakin 1B, also known as UPK1B, is a protein which in humans is encoded by the UPK1B gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The use of alternate polyadenylation sites has been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Membrane ; Single-pass type I membrane protein .
- Tissue details: Ubiquitous. Highly expressed in brain. .
- Research category: Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Tumor Biomarkers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
