Anti-Uroplakin3a UPK3A Monoclonal Antibody

Overview

This product is an anti-UPK3A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFEH-21; isotype Rabbit IgG; reactivity: Human. Reported application contexts include IHC, Flow (as provided in the source record). Boster Bio Anti-Uroplakin3a UPK3A Monoclonal Antibody catalog # M06534-1. Tested in IHC, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: UPK3A (Death domain-containing protein CRADD).
• Antibody format: Monoclonal; clone AFEH-21; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

UPK3A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Reported cellular localization context: Cytoplasm . Nucleus . Tissue expression notes (as provided): Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Research relevance and current trends

• Research context keywords from the source record include: Tags & Cell Markers,Cell Type Markers,Tumor Associated.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate UPK3A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect UPK3A in FFPE tissue sections by immunohistochemistry, Quantify UPK3A-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 23 kDa; calculated MW: 22745 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 23 kDa
• Cellular localization (provided): Cytoplasm . Nucleus .
• Tissue details (provided): Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.