| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ubiquitin carboxyl-terminal hydrolase 16; Deubiquitinating enzyme 16; Ubiquitin thioesterase 16; Ubiquitin-processing protease UBP-M; Ubiquitin-specific-processing protease 16; USP16; MSTP039 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human v-Myb/MYBL1 recombinant protein (Position: K199-L752). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-v-Myb/MYBL1 Antibody Picoband® is an antibody for MYBL1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: MYBL1 (ubiquitin specific peptidase 16); UniProt: P10243
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 86 kDa, calculated 16693 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-v-Myb/MYBL1 Antibody Picoband® catalog # A05803-2.
Biological background
Biological context: Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
Expression and localization notes: cellular localization: Nucleus., tissue context: Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas..
Common research applications
- Western blotting (WB): Compare MYBL1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of MYBL1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify MYBL1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: MYB proto-oncogene like 1 is a protein that in humans is encoded by the MYBL1 gene. A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes. Both A-Myb and B-Myb are expressed in t (14:18) lymphoma cells where they then inhibit cell arrest and apoptotic signaling. Expression of B-Myb rescues cells from p53-induced G1 phase arrest that is mediated by p21, while A-Myb functions as an anti-apoptotic factor by effectively activating the bcl-2 promoter and thereby up-regulating Bcl-2 expression.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Research category: Cancer,Cancer Susceptibility,Chromatin Binding Proteins,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Oncoproteins,Oncoproteins/Suppressors,Proto-Oncogenes,Transcription,Transcription Factors
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
