| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD309;KDR;FLK1, VEGFR2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets vascular endothelial growth factor receptor 2 (gene KDR) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 151.5 kDa, and the supplier reports an observed band at about 80 kDa.
VEGFR2 (KDR, also called FLK1) is a receptor tyrosine kinase on endothelial cells that binds VEGF-A as well as processed VEGF-C and VEGF-D. Ligand binding triggers receptor dimerization and autophosphorylation, activating PLCγ, PI3K/AKT and MAPK signaling that drives endothelial proliferation, migration, survival and vascular permeability. VEGFR2 is the main mediator of VEGF-driven angiogenesis and a major target of anti-angiogenic cancer drugs.
| Target | Vascular endothelial growth factor receptor 2 (gene KDR; UniProt P35968, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 151.5 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): SMMC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide from the middle region of human VEGF Receptor 2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places vascular endothelial growth factor receptor 2 in the cell junction, endoplasmic reticulum and cell membrane. Tissue expression noted by UniProt (human): Detected in cornea (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
