| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human VGLUT2/SLC17A6 recombinant protein (Position: M1-K561). Human SLC17A6 shares 98.4% and 98.6% amino acid (aa) sequence identity with mouse and rat SLC17A6, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-VGLUT2/SLC17A6 Antibody Picoband® is an antibody targeting SLC17A6. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: IgG; reactivity: Human,Mouse,Rat; observed MW: 64 kDa; calculated MW: 64 kDa.
Boster Bio Anti-VGLUT2/SLC17A6 Antibody Picoband® catalog # A04376-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: SLC17A6
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 64 kDa; Calculated: 64 kDa
Biological background
Scientific background (datasheet): Solute carrier family 17 (vesicular glutamate transporter), member 6 is a protein that in humans is encoded by the SLC17A6 gene. The SLC17A6 gene, also known as vesicular glutamate transporter 2 (VGLUT2), encodes a protein responsible for transporting the neurotransmitter glutamate into synaptic vesicles in neurons. This process is crucial for glutamatergic neurotransmission, which plays a fundamental role in synaptic communication and neuronal excitability. SLC17A6/VGLUT2 is primarily expressed in excitatory neurons in various regions of the brain, including the cerebral cortex, hippocampus, and thalamus. Dysregulation of SLC17A6 expression or function has been implicated in neurological disorders such as epilepsy, schizophrenia, and Parkinson's disease, highlighting its significance in brain function and disease pathology. Understanding the molecular mechanisms underlying SLC17A6/VGLUT2 function is essential for unraveling its role in normal synaptic transmission and its potential as a therapeutic target for neurological disorders.
Research relevance and current trends
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
