| Field | Specification |
|---|---|
| Alternative Names | Vimentin;Vim; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Pig eye lens vimentin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Vimentin Antibody (Monoclonal, V9) is an antibody targeting VIM. Common applications include WB, IHC, IF. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: V9; isotype: Mouse IgG1; reactivity: Human,Pig,Rabbit,Rat; observed MW: 54 kDa; calculated MW: 53733 MW.
Boster Bio Anti-Vimentin Antibody (Monoclonal, V9) catalog # MA1102. Tested in IF, IHC, WB applications. This antibody reacts with Human, Pig, Rabbit, Rat.
Key elements and design rationale
- Target: VIM — Vimentin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: V9; Isotype: Mouse IgG1
- Species reactivity: Human,Pig,Rabbit,Rat
- Molecular weight guidance: Observed: 54 kDa; Calculated: 53733 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. .
Scientific background (datasheet): The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.
Cellular localization (datasheet): Nucleus matrix . Cytoplasm .
Tissue details (datasheet): VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Neuroscience,Signal Transduction,Stem Cells,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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