| Field | Specification |
|---|---|
| Target | |
| Alternative names | VIP peptides;Intestinal peptide PHV-42;Peptide histidine valine 42;Intestinal peptide PHM-27;Peptide histidine methioninamide 27;Vasoactive intestinal peptide;VIP;Vasoactive intestinal polypeptide;VIP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human VIP, different from the related mouse and rat sequences by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes VIP peptides in human, mouse and rat samples and is validated for ICC/IF and IHC. The predicted molecular weight is 19.2 kDa.
Vasoactive intestinal peptide (VIP) is a neuropeptide produced in the central and peripheral nervous systems. Acting through the receptors VPAC1 and VPAC2, it relaxes smooth muscle, dilates blood vessels, stimulates intestinal secretion and modulates immune cells. VIP also helps synchronize the circadian clock in the suprachiasmatic nucleus.
| Target | VIP peptides (gene VIP; UniProt P01282, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 19.2 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Rat) |
Samples with confirmed staining (IHC): human pancreatic cancer tissue, rat pancreas tissue, rat intestine tissue, mouse pancreas tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): rat colon tissue.
Immunogen
Synthetic peptide from the middle region of human VIP. It differs from the mouse and rat sequences at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes VIP peptides as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kari R. Hoyt et al. (2026) Cellular- and systems-level profiling of amyloid-beta effects on circadian timing. Neurobiology of Disease. 10.1016/j.nbd.2026.107389
Huang Jinyu et al. (2013) S100+ cells: A new neuro-immune cross-talkers in lymph organs. Scientific Reports. 10.1038/srep01114
Ying Wang et al. (2025) Dynamic Regulation of Vasoactive Intestinal Polypeptide in the Retina of Guinea Pigs with Experimentally Induced Myopia. Experimental Eye Research. 10.1016/j.exer.2025.110728
Ayumi Sato et al. (2022) Innervation of the human minor salivary glands; immunohistochemical study for neurochemical substances. Tissue & Cell. 10.1016/j.tice.2022.101934
Mutsuko Kawashima et al. (2021) Parasympathetic neurons in the human submandibular ganglion. Tissue & Cell. 10.1016/j.tice.2021.101496
Tadasu Sato et al. (2021) Distribution of neuronal cells which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human internal carotid nerve. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.24755
Marianna Boi et al. (2024) The human major sublingual gland and its neuropeptidergic and nitrergic innervations. Annals of Anatomy-Anatomischer Anzeiger. 10.1016/j.aanat.2024.152291
Keiichiro Atsumi et al. (2020) Sensory neurons in the human jugular ganglion. Tissue & Cell. 10.1016/j.tice.2020.101344
Wang Ying et al. (2024) The role of vasoactive intestinal peptide (VIP) in atropine-related inhibition of the progression of myopia. BMC Ophthalmology. 10.1186/s12886-024-03309-9
Souichi Kokubun et al. (2019) Distribution of postganglionic neurons which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human middle cervical ganglion. Tissue & Cell. 10.1016/j.tice.2019.04.006
