| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Vasoactive intestinal polypeptide receptor 1; VIP-R-1; Pituitary adenylate cyclase-activating polypeptide type II receptor; PACAP type II receptor; PACAP-R-2; PACAP-R2; VPAC1; VIPR1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, which shares 84% amino acid (aa) sequence identity with both mouse and rat VIP Receptor 1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of VIPR1 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, which shares 84% amino acid (aa) sequence identity with both mouse and rat VIP Receptor 1.
- Molecular weight context: reported MW: 52 kDa; calculated MW: nan
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
vasoactive intestinal peptide receptor 1. VIPR1 (Vasoactive intestinal polypeptide receptor 1),also known as VIPR,HVR1, is a protein that in humans is encoded by the VIPR1 gene. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear specific high-affinity G protein-coupled receptors for VIP, such as VIPR1. The VIPR1 gene is mapped on 3p22.1. The VIPR1 gene was found to span approximately 22 kb and to be comprised of 13 exons (ranging from 42 to 1,400 bp) and 12 introns (ranging from 0.3 to 6.1 kb). One encodes a VIP receptor consisting of 460 amino acids and having 7 putative transmembrane domains, as do other G protein-coupled receptors. Patients with idiopathic achalasia show a significant difference in the distribution of SNPs affecting VIPR1. Functional note: PACAP-27 > PACAP-38. Reported localization: Cell membrane; Multi-pass membrane protein. Expression/tissue context: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT.
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how VIPR1 relates to this theme using model systems and orthogonal readouts.
- B Cells: Researchers commonly examine how VIPR1 relates to this theme using model systems and orthogonal readouts.
- Hematopoietic Progenitors: Researchers commonly examine how VIPR1 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative VIPR1 levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of VIPR1 across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
