| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Chromaffin granule amine transporter; Solute carrier family 18 member 1; Vesicular amine transporter 1; VAT1; SLC18A1; VAT1; VMAT1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human VMAT1/SLC18A1 recombinant protein (Position: M1-E510). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-VMAT1/SLC18A1 Antibody Picoband® is an antibody for SLC18A1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SLC18A1 (solute carrier family 18 member A1); UniProt: P54219; NCBI Gene: 6570
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 56 kDa, calculated 49229 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-VMAT1/SLC18A1 Antibody Picoband® catalog # A07692-1.
Biological background
Biological context: Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.
Expression and localization notes: cellular localization: Cytoplasmic vesicle membrane. Multi-pass membrane protein. Synaptic vesicle membrane. Multi-pass membrane protein. Endoplasmic reticulum membrane. Multi-pass membrane protein., tissue context: Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level)..
Common research applications
- Western blotting (WB): Compare SLC18A1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SLC18A1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SLC18A1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Chromaffin granule amine transporter is a protein that in humans is encoded by the SLC18A1 gene. This gene is mapped to 8p21.3. The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasmic vesicle membrane. Multi-pass membrane protein. Synaptic vesicle membrane. Multi-pass membrane protein. Endoplasmic reticulum membrane. Multi-pass membrane protein.
- Tissue details: Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).
- Research category: Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
