| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vacuolar protein sorting-associated protein 41 homolog; S53; VPS41 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human VPS41 recombinant protein (Position: Q44-R843). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes vacuolar protein sorting-associated protein 41 homolog (gene VPS41) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 98.6 kDa, and the supplier reports an observed band at about 99 kDa.
VPS41 is a subunit specific to the HOPS tethering complex, which mediates docking and fusion of late endosomes, autophagosomes and lysosomes. It also helps deliver lysosomal membrane proteins and supports regulated secretion in some cells. VPS41 is studied in lysosome biology and neurodegeneration.
| Target | Vacuolar protein sorting-associated protein 41 homolog (gene VPS41; UniProt P49754, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 98.6 kDa |
| Observed MW | 99 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL/ml (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human RT4 cells, human SH-SY5Y cells, rat liver tissue, mouse small intestine tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human VPS41 fragment (Gln44–Arg843), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places vacuolar protein sorting-associated protein 41 homolog in the endosome membrane, late endosome membrane, early endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Expressed in cerebral cortex and cerebellum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
