Anti-WAPL/FOE Antibody Picoband®

Overview

Anti-WAPL/FOE Antibody Picoband® is an antibody for WAPL detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: WAPL (selectin P ligand); UniProt: Q7Z5K2
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 180 kDa, calculated 28461 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-WAPL/FOE Antibody Picoband® catalog # A03684-2.

Biological background

Biological context: A SLe (x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

Expression and localization notes: cellular localization: Integral component of membrane., tissue context: Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach..

Common research applications

• Western blotting (WB): Compare WAPL levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of WAPL in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify WAPL-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth. It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by ly interacting with its regulatory subunits. WAPL is a new regulator of the development and metastasis of cancerous tissue.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Integral component of membrane.
• Tissue details: Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach.
• Research category: Cardiovascular,Cell Biology,Platelets,Protease Inhibitors,Proteolysis/Ubiquitin,Proteolytic Enzymes

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.