| Field | Specification |
|---|---|
| Target | |
| Alternative names | Wiskott-Aldrich syndrome protein family member 2;WASP family protein member 2;Protein WAVE-2;Verprolin homology domain-containing protein 2;WASF2;WAVE2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human WASF2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FFG-23 is a rabbit monoclonal antibody that recognizes actin-binding protein WASF2 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 54.3 kDa, and the supplier reports an observed band at about 90 kDa.
WAVE2 (WASF2) is a widely expressed member of the WASP/WAVE family and part of the WAVE regulatory complex. Activated downstream of Rac, it stimulates the Arp2/3 complex to build branched actin networks that drive lamellipodia at the leading edge of migrating cells. It is studied in cell motility and cancer cell invasion.
| Target | Actin-binding protein WASF2 (gene WASF2; UniProt Q9Y6W5, human) |
|---|---|
| Clone | FFG-23 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.3 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with confirmed staining (IHC): human colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human WASF2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places actin-binding protein WASF2 in the cytoplasm, cell projection and basolateral cell membrane. Tissue expression noted by UniProt (human): Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
