| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; p65; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1; WNK1; HSN2; KDP; KIAA0344; PRKWNK1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human WNK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19W36 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase WNK1 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 250.8 kDa, and the supplier reports an observed band at about 251 kDa.
WNK1 is a serine/threonine kinase of the "with no lysine" (WNK) family that activates the kinases SPAK and OSR1, which in turn phosphorylate Na-K-Cl and K-Cl cotransporters to control ion movement across membranes. It regulates cell volume and renal salt handling, and gene changes that raise WNK1 expression cause an inherited form of hypertension (pseudohypoaldosteronism type II).
| Target | Serine/threonine-protein kinase WNK1 (gene WNK1; UniProt Q9H4A3, human) |
|---|---|
| Clone | 19W36 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 250.8 kDa |
| Observed MW | 251 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): Saos-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human WNK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase WNK1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
