| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type II cytoskeletal 8;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;KRT8;CYK8; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human WNK1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-WNK1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 19W36; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-WNK1 Rabbit Monoclonal Antibody catalog # M01422-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: WNK1 (Keratin, type II cytoskeletal 8).
- Antibody format: Monoclonal; clone 19W36; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
WNK1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. . Reported cellular localization context: Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix . Tissue expression notes (as provided): Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. .
Research relevance and current trends
- Research context keywords from the source record include: Class I,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Keratin,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate WNK1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect WNK1 expression by Western blot in cell or tissue lysates, Detect WNK1 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 250 kDa; calculated MW: 53704 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 250 kDa
- Cellular localization (provided): Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
- Tissue details (provided): Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
