| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein Wnt-5a;WNT5A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Wnt5a |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABEE-23 is a rabbit monoclonal antibody that recognizes protein Wnt-5a (gene WNT5A) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 45 kDa.
Wnt-5a is a secreted Wnt ligand best known for non-canonical signaling, acting through receptors such as ROR2 and Frizzled to regulate cell polarity and migration. Depending on receptor context it can either activate or oppose β-catenin signaling, and it is required for outgrowth of the body axis, limbs and genitalia, so it is studied in development, inflammation and cancer.
| Target | Protein Wnt-5a (gene WNT5A; UniProt P41221, human) |
|---|---|
| Clone | ABEE-23 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.3 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human wnt5a.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes protein Wnt-5a as a secreted protein. Tissue expression noted by UniProt (human): Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenxia Li et al. (2025) Di-(2-ethylhexyl) Phthalate (DEHP) and Its Metabolite Promote Multiple Myeloma Progression and Bortezomib Resistance. Journal of Hazardous Materials. 10.1016/j.jhazmat.2025.139147
Wang Yuanyuan et al. (2024) MiR26a reverses enzalutamide resistance in a bone-tumor targeted system with an enhanced effect on bone metastatic CRPC. Journal of Nanobiotechnology. 10.1186/s12951-024-02438-z
Liu Yu-Biao et al. (2024) Wnt5a-mediated autophagy contributes to the epithelial-mesenchymal transition of human bronchial epithelial cells during asthma. Molecular Medicine. 10.1186/s10020-024-00862-3
