Anti-XRCC2 Antibody Picoband®

Overview

Anti-XRCC2 Antibody Picoband® is an antibody for Xrcc2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: Xrcc2 (X-ray repair complementing defective repair in Chinese hamster cells 2); UniProt: Q9CX47; NCBI Gene: 57434
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 36 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-XRCC2 Antibody Picoband® catalog # A02138-2.

Biological background

Biological context: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

Expression and localization notes: cellular localization: Nucleus., tissue context: Expressed at low level in somatic tissues and at high level in testis..

Common research applications

• Western blotting (WB): Compare Xrcc2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of Xrcc2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify Xrcc2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: DNA repair protein XRCC2 is a protein that in humans is encoded by the XRCC2 gene.This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus.
• Tissue details: Expressed at low level in somatic tissues and at high level in testis.
• Research category: Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.